1379 Cyclosporin A suppresses TGF-β2 expression via calcineurin/NFAT pathway in human dermal papilla cells

[Background and Objective] Hair cycle is a highly regulated cyclical process. In this cycle, three phases were defined; anagen (growing phase), catagen (regressing phase) telogen (resting phase). Hypertrichosis well-known side effect in patients receiving an immunosuppressant, cyclosporin A (CsA). CsA also elongates organ culture system of human hair follicles. Therefore, thought to cause hypertrichosis by elongation. However, the mechanism still unclear. immunosuppression, cyclophilin (CyP) form complex, complex inhibits for phosphatase activity calcineurin (CaN). Then, CaN stimulates translocation nuclear factor activated T-cells (NFAT) into nucleus. The objective study determine how causes hypertrichosis. [Methods] Immortalized dermal papilla cells (DPCs) used. DPCs cultured 10% FBS-DMEM. For evaluation compounds on gene expression, DMEM without FBS. was used as inhibitor, furthermore, VIVIT weas NFAT inhibitor. Evaluation expression carried out PCR or real-time PCR. measured using Calcineurin Phosphatase Activity Assay Kit (Abcam, ab139464). Intracellular calcium ion concentration Calcium Kit-Fluo 4 (Dojindo). [Results Conclusion] It confirmed that CyPs, CaN-A, CaN-B NFATs which are key molecules immunosuppression expressed DPCs. DPCs, TGF-β2 catagen-inducing suppressed VIVIT, but it increased addition chloride. Increased intracellular however, inactivated CaN. These results may suggest CaN/NFAT pathway involved suppression CsA.